PLATE COUNT AGAR TRYPTONE GLUCOSE YEAST AGAR
CM0325
Add 17.5g to 1 litre of distilled water. Dissolve by bringing to the boil with frequent stirring, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
PLATE COUNT AGAR
TRYPTONE GLUCOSE YEAST AGAR
Code: CM0325
A medium for the enumeration of viable organisms in food, animal feed and water samples
| Typical Formula* |
gm/litre |
| Tryptone |
5.0 |
| Yeast extract |
2.5 |
| Glucose |
1.0 |
| Agar |
9.0 |
| pH 7.0 ± 0.2 @ 25°C |
Description
Plate Count Agar is equivalent to the medium recommended by the APHA1, PHLS 2 and ISO3,4 for the plate count of micro-organisms in food.
The APHA method consists of the preparation of pour plates using diluted samples, and counting colonies after incubation. Incubation is for 48 hours at 32°C or at 35°C for the Standard Plate Count. For the enumeration of micro-organisms with other temperature requirements, plates may also be incubated for 7-10 days at 5-7°C: for 3-5 days at 20°C; for 2-3 days at 45°C; or for 48 hours at 55°C. See the APHA1 publication for details.
Samples should be tested following the method described in the appropriate standard.
The quality control of Plate Count Agar includes testing in accordance with ISO 11133:20145.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
