Distributor/Supplier/Penyedia/Agen/Jual M.R.S BROTH (DE MAN, ROGOSA, SHARPE) OXOID

MRS BROTH (DE MAN, ROGOSA, SHARPE)

CM0359

Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

MRS BROTH (DE MAN, ROGOSA, SHARPE)

Code: CM0359

A non-selective medium for profuse growth of `lactic acid bacteria’.

Typical Formula*	gm/litre
Peptone	10.0
`Lab-Lemco’ powder	8.0
Yeast extract	4.0
Glucose	20.0
Sorbitan mono-oleate	1 ml
Dipotassium hydrogen phosphate	2.0
Sodium acetate 3H₂O	5.0
Triammonium citrate	2.0
Magnesium sulphate 7H₂O	0.2
Manganese sulphate 4H₂O	0.05
pH 6.2 ± 0.2 @ 25°C

Description
MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith¹.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.