MRS BROTH (DE MAN, ROGOSA, SHARPE)
CM0359
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
MRS BROTH (DE MAN, ROGOSA, SHARPE)
CM0359
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
MRS BROTH (DE MAN, ROGOSA, SHARPE)
Code: CM0359
A non-selective medium for profuse growth of `lactic acid bacteria’.
Typical Formula* | gm/litre |
Peptone | 10.0 |
`Lab-Lemco’ powder | 8.0 |
Yeast extract | 4.0 |
Glucose | 20.0 |
Sorbitan mono-oleate | 1 ml |
Dipotassium hydrogen phosphate | 2.0 |
Sodium acetate 3H2O | 5.0 |
Triammonium citrate | 2.0 |
Magnesium sulphate 7H2O | 0.2 |
Manganese sulphate 4H2O | 0.05 |
pH 6.2 ± 0.2 @ 25°C |
Description
MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith1.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
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