NUTRIENT AGAR
CM0003 (Powder)
Suspend 28g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
NUTRIENT AGAR
Code: CM0003 (Powder)
A general purpose medium which may be enriched with up to 10% blood or other biological fluid.
| Typical Formula * | gm/litre |
| `Lab-Lemco’ powder | 1.0 |
| Yeast extract | 2.0 |
| Peptone | 5.0 |
| Sodium chloride | 5.0 |
| Agar | 15.0 |
| pH 7.4 ± 0.2 @ 25°C |
Description
Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.
In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms1.
Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required. The medium, without additions, may be used for the cultivation of organisms which are not exacting in their nutritional requirements.
